The blog covers issues broadly related to sustainability in Minnesota and the world both from an environmental perspective and from an interpersonal perspective. It also covers issues related to mycology (the study of fungi) with a specific emphasis on the interaction with fungal organisms and buildings.
The wood decay fungi, Taeniolella spp., is an anomorphic (asexual fungi) in the family Mytilinidiaceae. The organisms is often found indoors on water damaged or moist lumber and outdoors on leaves and wood. The organism does not grow well on typical culture media. This organism was identified from a tape lift of lumber above a crawlspace.
Taeoniolella spp. in sequence - N. Carlson
The organism can be visually similar to Alternaria spp. The Alternaria spp. spores are often in chains but are more club shaped with rounded edges at the end opposite the point of attachment.
Our local Ax-Man surplus store had a box of 36 prepared microscope slides. There were three starch particles. Two were easy to photograph. The foldscope was attached to a Samsung Galaxy 5 phone. Overhead fluorescent light provided the back lighting.
Wheat starch - foldscope photo - N. Carlson
Photoshop enhance image of wheat starch - N. Carlson
Potato starch - foldscope photo - N. Carlson
Photoshop enhanced image of potato starch - N. Carlson
Pachnocybe photo micrograph - N. Carlson - click to enlarge
I recently came across this basidiomycete fungi while in a crawlspace under a house. The organism was growing on the surface of the wood rim joist. The organism is much longer than the 250 micron guide on the photo. Fortunately the organism folded over on the tape lift allowing the whole structure to be photographed. The tape lifts were stained with lacto-fuchsin mixed with 85% lactic acid.
Ash and Lightning above an Icelandic Volcano Image Credit & Copyright: Sigurður Stefnisson
UK scientists have correlated the slower than expected rise in global temperatures to ash produced by volcanic activity. The Daily Mail report suggests that the impact of volcanic emissions had been underestimated in global climate models.
Geoengineers have proposed methods of resetting the planet's thermostat. One of the proposals attempts to mimic the the effects of a volcanic eruption. A series of volcanic eruptions are thought to have caused the little ice age from 1300 to 1500. More than 600 million years ago volcanoes are thought to have the opposite effect, warming the planet due to production of carbon dioxide.
Using a volcano to affect climate change is part of the premise of my fiction book set in 2028, The Smell of Truthiness.
Volcanic production of carbon dioxide helps end ice age over 600 million years ago - 3 min.
Tease tape samples were taken from this plate using 3M red tartan clear Scotch tape with Lacto Fuchsin stain in 85% lactic acid mounting fluid. Camera Samsung S-5. The Foldscope was back lit with a light for a dissecting scope at an approximate distance of one foot. Click on any of these images to enlarge.
Foldscope attached to Samsung S5 camera - side view
Magnetic pin hole strip double taped to the back of the camera
Foldscope attached to camera - front view
Foldscope image with medium pinhole magnification - image cropped with Adobe Photoshop Elements
Uncropped higher magnification pin hole Foldscope image of Aspergillus ochraceous - mature vesicle with spores
Aspergillus ochraceous image cropped with Adobe Photoshop Elements
The two day Mold Remediation course covers health effects, investigation and methods of finding and identifying mold including use of moisture meters and infrared cameras. The second day focuses on mold remediation including a hands on demonstration setting up a containment and wearing PPE.
The photos below are from fungal growth in a walk in cooler. I wanted to determine if the Foldscope could be used for field identification of a fungal organism. All photos except the picture in the cold room were taken with a Samsung Galaxy S-5 smart phone camera. Room florescent lights were used to back light the Foldscope image.
Possible fungal growth on a metal shelf in a walk in cooler
Foldscope photo using 85% lactic acid with lacto fuchsin stain and 3M scotch tape on microscope slide
Digitally enhanced Foldscope photo of Cladosporium sphaerospermum with Adobe Photoshop Elements 12
Original photo from the eyepiece of a Olympus compound microscope at 400x
Digitally cropped Olympus microscope photo with Photoshop Elements 12.
Conclusion: The $1 Foldscope microscope gives me enough information to field identify Cladosporium spp. After the shot is taken with the camera it can be viewed and enlarged on the camera photo viewer. The image will not be as sharp as the Photoshop enhanced image or that produced by a Nikon microscope but it provides enough information to make the identification.
Stanford University developed a low cost (less than $1) foldoscope microscope for field identification of microscopic particles of around 5 microns or larger. The scope comes with an attachment that can be affixed to a cellphone camera. I received two of them in the mail in December of 2014.
The first foldoscope took about 1/2 hour to assemble based on instructions on the sheet. The second camera took 15 minutes as I grew more familiar with the assembly procedure.
A sample can be taken and placed on a slide provided or a standard microscope slide. The foldoscope can be held up to a room light to see the image.
Below are photos taken with a Google Nexus 5 camera phone. The first photo is the original. The second photo is cropped with contrast and sharpening using Adobe Photoshop Elements 12 software.
Same image cropped, contrast improved and sharpened - click to enlarge
Conclusion: It takes a bit of work to focus the camera image and a bit of photo editing but for less than one $1 US, it is possible to identify a fungal organism down to the genus level.
Foldoscope: Manu Prakash - Stanford University - 15 min.
to identify the organism. 
