Thursday, December 20, 2012

Aspergillus fumigatus

The thermophillic fungus, Aspergillus fumigatus, aids in decomposing compost piles and can also cause pulmonary aspergillosis in immune compromised individuals. 

It grows rapidly at 37 degrees Celsius on PDA and MEA with a flat blue green colony that can cover the plate in less than 3 days.  Growth is somewhat slower at 25 degrees on PDA and MEA.   On DG-18 the growth of the colony is restricted often less than 1 cm in 5 days. 

Unless the actual head of the Aspergillus fumigatus spore is found on an Air-O-Cell cassette sample it is not possible to identify the spore other than to put it in the category of Aspergillus/Penicillium like organism.

The spores are small, typically less than 3 microns.  The spores cover the  top 2/3rds of the vesicle.  The stipe of the conidiophore is smooth and often twisted.

Aspergillus fumigatus - line drawing - N. Carlson
 
Aspergillus fumigatus - lactophenol cotton blue stain - N. Carlson
Aspergillus fumigatus - 25C DG-18 - N. Carlson

Aspergillus fumigatus - MEA 25C in culture - N. Carlson

 
 
Aspergillus fumigatus 400x 85% lactic acid stain - N. Carlson
 
Aspergillus fumigatus 100x stereoscope lit from the side - N. Carlson
 
 
Video on Aspergillosis by the IAQ Network

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