Fungal mounting fluid stain recipes

Two rather simple stains make it easier to see the cell walls when preparing a slide to view under a microscope

Lacto-Fuchsin

Add 0.1 grams of acid fuchsin stain to 100 milliliters of 85% lactic acid.  On occasion I find this stain may be a bit strong and diluted it 1/10 with 85% lactic acid. 

Reference: Charmichael, J.W. 1955. Lacto-fuchsin a new medium for mounting fungi. Mycologia 4:611.

85% Lactic Acid

Straight 85% lactic acid is useful for getting a true color image of the fungal spore and structures without staining.  This is helpful for some of the darker colored organisms.

Stacybotrys spp. and Ulocladium spp. lacto fuchsin stain

Stacybotrys spp.  85% lactic acid